R 15.4 cells. To do so, we designed a modified quantitative RTPCR (RT-qPCR). Our modified method, herein named RT-DNA-qPCR (RT-DqPCR), is based on the fact that molar ratio between two genes in genomic DNA is equal (see Supplemental text 1). In short, RT-DqPCR involves normalization of values obtained by amplification of cDNA to those obtained by amplification of genomic DNA with the same primer p
Es obtained with the LTR-specific primers represent a sum of transcripts originating from the host promoter and of viral transcripts, which also contain LTR sequence at their 3' ends. Therefore, the measured ratio corresponds to the equation a+b/b, where `a' is the number of host-viral chimeric transcripts and `b' the number of viral transcripts (see Supplemental text 2). First, we determined this
R 15.4 cells. To do so, we designed a modified quantitative RTPCR (RT-qPCR). Our modified method, herein named RT-DNA-qPCR (RT-DqPCR), is based on the fact that molar ratio between two genes in genomic DNA is equal (see Supplemental text 1). In short, RT-DqPCR involves normalization of values obtained by amplification of cDNA to those obtained by amplification of genomic DNA with the same primer p
Es obtained with the LTR-specific primers represent a sum of transcripts originating from the host promoter and of viral transcripts, which also contain LTR sequence at their 3' ends. Therefore, the measured ratio corresponds to the equation a+b/b, where `a' is the number of host-viral chimeric transcripts and `b' the number of viral transcripts (see Supplemental text 2). First, we determined this
Es obtained with the LTR-specific primers represent a sum of transcripts originating from the host promoter and of viral transcripts, which also contain LTR sequence at their 3' ends. Therefore, the measured ratio corresponds to the equation a+b/b, where `a' is the number of host-viral chimeric transcripts and `b' the number of viral transcripts (see Supplemental text 2). First, we determined this
Tream of the 5'LTR Existence of the host-viral chimeric transcripts that originate from a host promoter and include 5'LTR could be useful for identifying ongoing transcription upstream of the 5'LTR independently of the site of viral integration. Therefore, we next devised an assay, which determines the ratio between transcripts containing LTR sequences and those containing sequences present solely
R 15.4 cells. To do so, we designed a modified quantitative RTPCR (RT-qPCR). Our modified method, herein named RT-DNA-qPCR (RT-DqPCR), is based on the fact that molar ratio between two genes in genomic DNA is equal (see Supplemental text 1). In short, RT-DqPCR involves normalization of values obtained by amplification of cDNA to those obtained by amplification of genomic DNA with the same primer p
The prioritization of health interventions [22]. The trade-offs between efficiency and equity are among these criteria, and have long been emphasized in the field of HIV/AIDS treatment and prevention [23,24]. Several mathematical frameworks, including mathematical programming, have been proposed to incorporate equity considerations into resource allocation in the public sector [25-29]. Several of