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/ml, respectively made up to 200 l using Tris-EDTA buffer. The suspensions were vortexed for 10 s and incubated at room temperature for 10 min with 2 min mixing interval. RNA extraction of the treated cells was performed using RNeasy Plus Mini kit (Qiagen, Germany) according to manufacturer's guidelines and eluted in DEPC water (Bioline, UK). A total of three biological replicates were included f
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Morigenesis, mTOR signaling pathway being a major pathway identified. Comparison of 340 differentially expressed proteins with the transcript data from Grade II diffuse astrocytomas reported earlier, revealed about 190 of the proteins correlate in their trends in expression. Considering progressive and recurrent nature of these tumors, we have mapped the differentially expressed proteins for thei
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What makes tattoos permanent is that it goes deep in the second layer of skin which is sometimes called dermis.
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