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In 40 ml LB supplemented with 12.5 mg/ml tetracycline and 12.5 mg/ml carbenicillin and grown at 28uC, 180 rpm overnight. Eggs had been prepared from gravid C. elegans adults by alkaline sodium hypochlorite treatment and permitted to create in M9 at 15uC overnight. Day 2: 1.0 mM IPTG was added as well as the incubation was continued for a different 2 h at 37uC, 180 rpm. A suspension of synchronized

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